A microscope’s job is to magnify the minuscule world around us. We can observe the tiniest objects, organisms and materials, and investigate their form, texture and composition, to witness what would ...

Fundamentally, a microscope comprises two subsystems: an illumination system to illuminate the sample and an imaging system that produces a magnified image of the light that has interacted with the ...

This protocol outlines a procedure for collecting and analyzing point spread functions (PSFs). It describes how to prepare fluorescent microsphere samples, set up a confocal microscope to properly ...